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NR-14856   Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
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Description: Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14856 is a preparation of antigen 85A (Ag85A) protein purified from the culture filtrate proteins of Mycobacterium tuberculosis, strain H37Rv.

The culture filtrate proteins were precipitated from culture filtrate with 40% ammonium sulfate. The precipitate was suspended and applied to phenyl sepharose HPLC. Antigen 85 was obtained by increasing the pH and eluting with a high concentration of ethylene glycol. Fractionation of the Ag85 complex was performed by size exclusion chromatography to yield the Ag85A component. The purified product was dialyzed in 10 mM ammonium bicarbonate.

Each vial contains approximately 250 µg of lyophilized, purified Ag85A from Mycobacterium tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.

Note: NR-14856 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ag85A (Gene Rv3804c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-14856."
Publications Citing
this Reagent:
Ferrara, F., et al. "Using Phage and Yeast Display to Select Hundreds of Monoclonal Antibodies: Application to Antigen 85, A Tuberculosis Biomarker." PLoS One 7 (2012): e49535. PubMed: 23166701.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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