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  Question General Solubility for Mycobacterium products
Author: BEI Resources
Submitted on: 7/16/2013 12:30:34 PM
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  Answer
  • Glycolipids and total lipids are generally soluble in 2:1 chloroform:methanol or DMSO.
  • Lipoglycans are generally soluble in molecular-grade water or 100-500 mM aqueous buffered salt solutions.
  • Peptidoglycan and arabinoglactan may require bath sonication.
  • Cell Wall Core (mAGP) may be reconstituted in acetone. mAGP is poorly soluble and sonication is generally required to achieve a uniform suspension.
  • Mycolic acids may be resuspended in chloroform or dichloromethanol.
  • Proteins are generally soluble in 10 mM ammonium bicarbonate.
  • Subcellular fractions from from Mycobacterium tuberculosis, Mycobacterium canetti, and Mycobacterium bovis are provided frozen and may not fully solubilize, however a uniform suspension can be achieved with bath sonication.
  • Recombinant proteins from Mycobacterium leprae are generally soluble in 100-500 mM buffered salt solutions or 10 mM ammonium bicarbonate.
  • Lyophilized irradiated, genomic DNA, extracted protein pools and monoclonal antibodies cells from Mycobacterium leprae may be reconstituted in molecular-grade water.
  • Subcellular fractions from Mycobacterium leprae are provided lyophilized and are generally soluble in water or 100-500 mM buffered salt solutions.
  • Phenolic glycolipids from Mycobacterium leprae are soluble in 100% methanol or ethanol or 2:1 chloroform:methanol phenolic glycolipids are not soluble in aqueous buffers.

Solubility Notes: When appropriate, DMSO is the ideal solvent if the product will be used in downstream biological assays. When resuspending product, use a minimal volume of solvent and then dilute into buffer or cell culture medium for use in biological assays. For product-specific solubility and recommended solvents, please refer to the appropriate Product Information Sheet available on the BEI Resources website.
 
Last Updated: 7/16/2013 12:30:34 PM
 

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