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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-14859  Mycobacterium tuberculosis, PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Strain H37Rv
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Strain H37Rv
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14859 is a preparation of native protein, PstS1 (Gene Rv0934, non-acylated), purified from the culture filtrate of Mycobacterium tuberculosis, strain H37Rv. PstS1 was precipitated by ammonium sulfate, applied to ConA and phenyl sepharose columns, and eluted using a gradient of decreasing ammonium sulfate. Fractions containing PstS1 were pooled and dialyzed.

Note: The cell wall-associated and culture filtrate forms of PstS1 seem to differ in their hydrophobic characteristics, however a chemical difference between the two forms has not been determined.

Each vial contains approximately 250 µg of lyophilized, purified NR-14859 in 10 mM ammonium bicarbonate.

Note: NR-14859 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: PstS1 (Gene Rv0934, Non-Acylated), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-14859."
Publications Citing
this Reagent:
Macdonald, S. H., et al. "Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis." PLoS One 7 (2012): e38488. PubMed: 22675566.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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