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NR-14860  Mycobacterium tuberculosis, a-Crystallin (Gene Rv2031c), Purified Native Protein from Strain H37Rv
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: a-Crystallin (Gene Rv2031c), Purified Native Protein from Strain H37Rv
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14860 is a preparation of α-crystallin derived from the whole cell lysate of irradiated Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv. M. tuberculosis whole cell lysate was extracted with 0.1% n-octylthioglucoside and fractionated by isoelectric focusing. Fractions containing 16 kDa proteins were cleaned by size exclusion chromatography and dialyzed against 10 mM ammonium bicarbonate.

Each vial contains approximately 100 µg of lyophilized α-crystallin in 10 mM ammonium bicarbonate.

Note: NR-14860 is soluble in 100 mM to 500mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: α-Crystallin (Gene Rv2031c), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-14860."
Publications Citing
this Reagent:
Macdonald, S. H., et al. "Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis." PLoS One 7 (2012): e38488. PubMed: 22675566.

Marongiu, L., et al. "ESAT-6 and HspX Improve the Effectiveness of BCG to Induce Human Dendritic Cells-Dependent Th1 and NK Cells Activation." PLoS One 8 (2013): e75684. PubMed: 24130733.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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