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NR-19240   H1 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: H1 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus
Organism: Influenza A virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A recombinant form of the H1 hemagglutinin (HA) protein from influenza A virus, A/Puerto Rico/8/1934 (H1N1) containing a C-terminal histidine tag was produced in Sf9 insect cells using a baculovirus expression vector system and was purified by nickel affinity chromatography. The predicted protein sequence is shown in Table 1 on the Product Information Sheet. The HA protein includes a C-terminal peptide containing a thrombin cleavage site, trimerizing (foldon) domain and eight histidine residues, as described for the 1918 pandemic virus. The full-length HA precursor protein is 562 residues (GenPept: AEX92873).

Each vial contains approximately 50 to 100 µg of purified recombinant HA protein in PBS (pH 7.4) with 50% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: H1 Hemagglutinin (HA) Protein with C-Terminal Histidine Tag from Influenza Virus, A/Puerto Rico/8/1934 (H1N1), Recombinant from Baculovirus, NR-19240."
Publications Citing
this Reagent:
Nogales, A., et al. "Influenza A Virus Attenuation by Codon Deoptimization of the NS Gene for Vaccine Development." J. Virol. 88 (2014): 10525-10540. PubMed: 24965472.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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