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NR-21986  Vaccinia virus, Western Reserve, L1R Protein with C-Terminal Histidine Tag, Recombinant from Baculovirus
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Description: Western Reserve, L1R Protein with C-Terminal Histidine Tag, Recombinant from Baculovirus
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: GH Cohen, RJ Eisenberg
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

A recombinant form of the L1R membrane glycoprotein [L1R (185t)] of the Western Reserve (WR) strain of vaccinia virus containing a C-terminal histidine-tag was produced in High-5 insect cells using a baculovirus expression system and purified using nickel affinity chromatography. NR-21986 contains residues 1 to 185 of the L1R protein. The full length L1R protein is 250 residues (GenPept: P07612). NR-21986 was expressed from the same recombinant baculovirus vector as NR-2625, which was produced in cabbage looper (Trichoplusia ni) insect larvae.

NR-21986 was demonstrated to be functionally active based on its reactivity with human polyclonal anti-vaccinia virus immune globulin (VIG; BEI Resources NR-650) and mouse monoclonal antibodies to L1R (BEI Resources NR-417 to NR-421 and NR-566).

Each vial contains approximately 100 µg of NR-21986 in approximately 100 µL of PBS (pH 7.4) with 50% glycerol (v/v).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Vaccinia Virus (WR) L1R Protein with C-Terminal Histidine Tag, Recombinant from Baculovirus, NR-21986."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Vaccinia virus Western Reserve, L1R Protein with C-Terminal Histidine Tag, Recombinant from Baculovirus
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