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NR-44223   Fraction 1 Capsular Antigen (F1) from Yersinia pestis, Expressed in Escherichia coli
(Proteins)
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at no cost to registered researchers.
Description: Fraction 1 Capsular Antigen (F1) from Yersinia pestis, Expressed in Escherichia coli
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-44223 is a recombinant form of the Yersinia pestis (Y. pestis) fraction 1 capsular antigen (F1). F1 is a plasmid (pFra)-encoded proteinaceous capsule synthesized in large quantities by Y. pestis and reported to confer antiphagocytic properties on the pathogen by interfering with complement-mediated opsonization. The protein is highly immunogenic and has been indirectly associated with eliciting a protective immune response in humans. A recombinant protein lacking the native signal sequence but including an N-terminal hexa-histidine tag, a thrombin cleavage site, and amino acid residues 22 to 170 of F1 from Y. pestis (GenPept: AAS58714) was expressed in Escherichia coli and purified by nickel affinity chromatography. Treatment with immobilized bovine thrombin resulted in a protein with four non-F1 residues at the amino terminus. The thrombin-cleaved protein was further purified by anion exchange chromatography and another round of nickel affinity chromatography to remove the cleaved hexa-histidine tag. NR-44223 has a theoretical molecular weight of 15,976 daltons.

Each vial contains approximately 200 µg of NR-44223 in phosphate-buffered saline, pH 7.4.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Fraction 1 Capsular Antigen (F1) from Yersinia pestis, Expressed in Escherichia coli, NR-44223."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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