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Product Name:
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WGLW4
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Morganellaceae, Proteus (previously classified as Enterobacteriaceae)1
Species: Proteus mirabilis
Strain: WGLW4
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Additional Information:
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P. mirabilis is a Gram-negative, dimorphic, highly motile enterobacterium that is a frequent cause of urinary tract infections in patients with long-term indwelling catheters or with compromised (e.g., injured or abnormal) urinary tracts.4 Typical virulence factors are associated with adhesion, motility, immunoavoidance, nutrient acquisition, host damage, as well as biofilm formation.4 P. mirabilis has a unique ability to differentiate between short, vegetative cells and elongated, hyperflagellated, swarmer cells.4,5 Due to increasing drug resistance and severe complications in infection, there has been renewed interest in P. mirabilis vaccine development. Several complete genomic sequences are now available.5,6
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Nutrient broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-752 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Nutrient broth or Luria-Burtani (LB) broth or equivalent
Nutrient agar or Tryptic Soy agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Adeolu, M., et al. “Genome-Based Phylogeny and Taxonomy of the ‘Enterobacterales’: Proposal for Enterobacterales ord. nov. Divided into the Families Enterobacteriaceae, Erwiniaceae fam. nov., Pectobacteriaceae fam. nov., Yersiniaceae fam. nov., Hafniaceae fam. nov., Morganellaceae fam. nov., and Budviciaceae fam. nov.” Int. J. Syst. Evol. Microbiol. 66 (2016): 5575-5599. PubMed: 27620848.
2. Garrett, W. S. and L. H. Wardwell, Personal Communication.
3. Garrett, W. S., et al. “Enterobacteriaceae Act in Concert with the Gut Microbiota to Induce Spontaneous and Maternally Transmitted Colitis.” Cell Host Microbe 8 (2010): 292-300. PubMed: 20833380.
4. Armbruster, C. E. and H. L. T. Mobley. “Merging Mythology and Morphology: The Multifaceted Lifestyle of Proteus mirabilis.” Nat. Rev. Microbiol. 10 (2012): 743-754. PubMed: 23042564.
5. Pearson, M. M., et al. “Complete Genome Sequence of Uropathogenic Proteus mirabilis, a Master of Both Adherence and Motility.” J. Bacteriol. 190 (2008): 4027-4037. PubMed: 18375554.
6. Sullivan, N. L., et al. “The Complete Genome Sequence of Proteus mirabilis Strain BB2000 Reveals Differences from the P. mirabilis Reference Strain.” Genome Announc. 1 (2013): e00024-13. PubMed: 24009111.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Proteus mirabilis, Strain WGLW4, HM-752."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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