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Product Name:
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E2883
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterococcaceae,
Enterococcus
Species: Enterococcus faecium
Strain: E2883 (also referred to as EnGen00301)
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Additional Information:
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E. faecium is a Gram-positive, facultative anaerobic coccus that is a commensal inhabitant of the
gastrointestinal tract of both humans and animals.4,5,6 E. faecium is an emerging and
challenging nosocomial pathogen due to its inherent hardiness and ability to develop antibiotic resistance.4,6 Its large open pan-genome allows for horizontal gene transfer between E. faecium and other pathogenic and non-pathogenic bacteria to adapt to changing environments.4,7 The large majority of strains isolated from nosocomial infections have been classified as CC17, with a distinct genetic lineage characterized by ampicillin resistance and a pathogenicity island carrying the esp gene, which is known to contribute virulence in an animal model.4,7,8 Two other virulence genes, hyl and acm, have been identified.4
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-31955 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or Brain Heart Infusion broth or equivalent
Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or Brain Heart Infusion agar or equivalent
Incubation:
Temperature: 35°C to 37°C
Atmosphere: Aerobic (with or without 5% CO2) or anaerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate tube, slant and/or plate for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use.
Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org.
While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate.
This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products.
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References:
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1. Gilmore, M. S., Personal Communication.
2. Lebreton, F., et al. “Emergence of Epidemic Multidrug-Resistant Enterococcus faecium
from Animal and Commensal Strains.” mBio 4 (2013): e00534-13. PubMed: 23963180.
3. Gilmore, M. S. “Scope of the Diversity of Emerging Hospital Pathogens, Enterococcus faecalis and Enterococcus faecium.” Broad Institute. <https://www.broadinstitute.org/files/shared/genomebio/Enterococcal%20genomes%20whitepaper.doc>
4. van Schaik, W., et al. “Pyrosequencing-Based Comparative Genome Analysis of the Nosocomial Pathogen Enterococcus faecium and Identification of a Large Transferable Pathogenicity Island.” BMC
Genomics 11 (2010): 239. PubMed: 20398277.
5. Schleifer, K. H. and R. Kilpper-Bälz. "Transfer of Streptococcus faecalis and Streptococcus faecium to the Genus Enterococcus nom. rev. as Enterococcus faecalis comb. nov. and Enterococcus faecium comb. nov." Int.
J. Syst. Bacteriol. 34 (1984):
31-34.
6. Arias, C. A. and B. E. Murray. “The Rise of the Enterococcus: Beyond Vancomycin Resistance.” Nat. Rev. Microbiol. 10 (2012): 266-278. PubMed: 22421879.
7. Heikens, E., et al. “Identification of a Novel Genomic Island Specific to Hospital-Acquired Clonal Complex 17 Enterococcus faecium Isolates.” Appl. Environ. Microbiol. 74 (2008): 7094-7097.
PubMed: 18836023.
8. Willems, R. J., et al. “Global Spread of Vancomycin-Resistant Enterococcus faecium from Distinct Nosocomial Genetic Complex.” Emerg. Infect. Dis. 11 (2010): 821-828.
PubMed: 15963275.
9. Homan, W. L., et al. “Multilocus Sequence Typing Scheme for Enterococcus faecium.” J.
Clin. Microbiol. 40 (2002): 1963-1971. PubMed: 12037049.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Enterococcus faecium, Strain E2883, NR-31955."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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