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Product Name:
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ABC020063118
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Ownership statement:
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This reagent is the tangible property of the U.S. Government.
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Streptococcaceae, Streptococcus
Species: Streptococcus pyogenes
Strain: ABC020063118
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Additional Information:
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S. pyogenes is a non-motile, non-sporulating, Gram-positive, β-hemolytic coccus found in normal human nasopharyngeal flora and is one of the most frequent pathogens of humans. It is estimated that between 5 to 15% of normal individuals harbor S. pyogenes without signs of disease. Mild infections may present as pharyngitis (strep throat), scarlet fever (rash), impetigo (superficial skin) or cellulitis (deep skin). Invasive, toxigenic infections can result in necrotizing fasciitis, myositis and streptococcal toxic shock syndrome.2,3,4,5,6
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work. |
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Packing/Storage:
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NR-48702 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Tryptic Soy broth or Todd-Hewitt broth or equivalent
Tryptic Soy agar or Tryptic Soy agar with 5% sheep blood or Todd-Hewitt agar or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic with 5% CO2
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Van Beneden, C., Personal Communication.
2. Beres, S. B., et al. “Genome-Wide Molecular Dissection of Serotype M3 Group A Streptococcus Strains Causing Two Epidemics of Invasive Infections.” Proc. Natl. Acad. Sci. USA 101 (2004): 11833-11838. PubMed: 15282372.
3. Beres, S. B., et al. “Molecular Genetic Anatomy of Inter- and Intraserotype Variation in the Human Bacterial Pathogen Group A Streptococcus.” Proc. Natl. Acad. Sci. USA 103 (2006): 7059-7064. PubMed: 16636287.
4. Beres, S. B., et al. “Genome Sequence of a Serotype M3 Strain of Group A Streptococcus: Phage-Encoded Toxins, the High-Virulence Phenotype, and Clone Emergence.” Proc. Natl. Acad. Sci. USA 99 (2002): 10078-10083. PubMed: 12122206.
5. Davies, H. D., et al. “Invasive Group A Streptococcal Infections in Ontario, Canada. Ontario Group A Streptococcal Study Group.” N. Engl. J. Med. 335 (1996): 547-554. PubMed: 8684408.
6. Olsen, R. J. and J. M. Musser. “Molecular Pathogenesis of Necrotizing Fasciitis.” Annu. Rev. Pathol. 5 (2010): 1-31. PubMed: 19737105.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Streptococcus pyogenes, Strain ABC020063118, NR-48702."
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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