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Product Name:
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Ag2
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Serratia
Species: Deposited as Serratia sp. [NR-50123 aligns favorably with the depositors’ sequence, however, this organism did not align favorably with other members of the Serratia genus. Digital DNA-DNA hybridization (dDDH) analysis of the sequence was also inconclusive.]
Strain: Ag2
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Additional Information:
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Serratia species are Gram-negative, rod-shaped facultative anaerobes that exhibit swarming motility. Serratia sp. are ubiquitous in water, soil and plant surfaces and are also found in the guts of vertebrates and invertebrates.2,3,4 Serratia marcescens (S. marcescens), S. plymuthica and S. rubidae, produce prodigiosin, a characteristic non-diffusible, water- insoluble red pigment.3,4 These opportunistic pathogens are a rising cause of nosocomial infections in immunocompromised patients, mainly due to the formation of biofilms on catheters, other medical devices, and on contact lenses.3,4,5 Infection by Serratia is complicated by an inherent resistance to β-lactam antibiotics, attributed to the naturally occurring expression of the AmpC gene.4,5 Intrinsic resistance to macrolides (linezolid, glycopeptides, quinopristin-dalfopristin, rifampin and nitrofurantoin) has also been observed.4 Anti-malarial properties of Serratia species present in the mid-gut lumen of Anopheles mosquitoes have been reported.5
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Brain Heart Infusion broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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NR-50123 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided
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Growth Conditions:
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Media:
Tryptic Soy broth or Nutrient broth or Brain Heart Infusion broth or equivalent
Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 26°C to 30°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Xu, J., Personal Communication.
2. Pei, D., et al. “Draft Genome Sequences of Two Strains of Serratia spp. from the Midgut of the Malaria Mosquito Anopheles gambiae.” Genome Announc. 3(2015): e00090-15. PubMed: 25767231.
3. Grimont, F. and P. A. D. Grimont. “The Genus Serratia.” In: M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer and E. Stackebrandt (Eds.), The Prokaryotes: Handbook on the Biology of Bacteria. (Vol. 3) New York: Springer Verlag, 2006. 219-244.
4. Mahlen, S. D. “Serratia Infections: From Military Experiments to Current Practice.” Clin. Microbiol. Rev. 24 (2011): 755-791. PubMed: 21976608.
5. Stock, I., et al. “Natural Antimicrobial Susceptibilities of Strains of ‘Unusual’ Serratia Species: S. ficaria, S. fonticola, S. odorifera, S. plymuthica and S. rubidaea.” J. Antimicrob. Chemother. 51 (2003): 865-885. PubMed: 12654765.
6. Bando, H., et al. “Intra-Specific Diversity of Serratia marcescens in Anopheles Mosquito Midgut Defines Plasmodium Transmission Capacity.” Sci. Rep. 3 (2013): 1641. PubMed: 23571408.
7. Grimont, P. A. and F. Grimont. “The Genus Serratia.” Annu. Rev. Microbiol. 32 (1978): 221-248. PubMed: 360966.
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Citation:
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Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Serratia sp., Strain Ag2, NR-50123.”
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Biosafety Level:
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2
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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