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NR-19600   Yersinia pestis, Strain KIM, Gateway® Clone Set, Recombinant in Escherichia coli, Plate 4
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Description: Yersinia pestis, Strain KIM, Gateway® Clone Set, Recombinant in Escherichia coli, Plate 4
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: Pathogen Functional Genomics Resource Center at the J. Craig Venter Institute
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Yersinia pestis (Y. pestis), strain KIM, Gateway® clone set consists of 43 plates (plate 2 of this clone set has been discontinued) which contain more than 3600 sequence validated clones from Y. pestis, strain KIM cloned in Escherichia coli (E. coli) DH10B-T1 cells. Each open reading frame was constructed in vector pDONR™221 (Invitrogen™) with an ATG start codon and a TAG stop codon. The sequence was validated by full length sequencing of each clone with greater than 1X coverage and a mutation rate of less than 0.2%.

Information related to the use of Gateway® Clones can be obtained from Invitrogen™. Recombination was facilitated through an attB substrate (attB-PCR product or a linearized attB expression clone) with an attP substrate (pDONR™221) to create an attL-containing entry clone. The entry clone contains recombinational cloning sites, attL1 and attL2 to facilitate gene transfer into a destination vector, M13 forward and reverse priming sites for sequencing and a kanamycin resistance gene for selection. Please refer to the Invitrogen™ Gateway® Technology Manual for additional details.

Each inoculated well of the 96-well plate contains approximately 60 µL of E. coli culture (strain DH10B-T1) in Luria Bertani (LB) Broth containing 50 µg/mL kanamycin supplemented with 15% glycerol.

Note: Production in the 96-well format has increased risk of cross-contamination between adjacent wells. Individual clones should be purified (e.g. single colony isolation and purification using good microbiological practices) and sequence-verified prior to use. BEI Resources cannot confirm or validate any clone not identified on the plate information table found on the Product Information Sheet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Yersinia pestis, Strain KIM, Gateway® Clone Set, Recombinant in Escherichia coli, Plate 4, NR-19600."
Additional Information: NR-19600 JCVI Plate 04 Map
Limited Use Label License
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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