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NR-48767  Cryptococcus neoformans, H99O
(Fungi)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Cryptococcus neoformans
Designations: H99O
Biosafety Level: 2
Availability Status: In Stock
Store at: -130°C or colder
Contributor: J Heitman
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Cryptococcus neoformans (C. neoformans), strain H99 was isolated from the cerebrospinal fluid of a human male who had been treated for Hodgkin's disease in North Carolina, USA on February 14, 1978. After an unknown number of passages, strain H99 was reported to lose virulence and was subsequently passaged through the rabbit model of infection to increase its virulence. It was renamed as H99O and frozen in 1994.

C. neoformans var. grubii, strain H99O (H99) was sequenced in 2014 through a collaboration between Duke University and the Broad Institute (NCBI BioProject: PRJNA411). Strain H99O is the progenitor of nine phenotypic variants that display either increased or decreased mating ability, melanization and virulence.

BEI Resources Number    Strain Name    Mating Ability/Melanization Virulence
NR-48767 H99O N/A
NR-48768 KN99a Increased
NR-48769 KN99a Increased
NR-48770 H99F Increased
NR-48771 H99C Decreased
NR-48772 H99S Increased
NR-48773 H99W Decreased
NR-48774 H99ED Decreased
NR-48775 H99E Decreased
NR-48776 YL99a Increased
NR-48777 YL99a Increased

Each vial contains approximately 0.5 mL of C. neoformans in 20% glycerol.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Cryptococcus neoformans, Strain H99O, NR-48767."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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