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NR-10049  Staphylococcus aureus subsp. aureus, Staphylococcal Enterotoxin B Toxoid, Recombinant from Escherichia coli
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Staphylococcus aureus subsp. aureus
Description: Staphylococcal Enterotoxin B Toxoid, Recombinant from Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -70°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-10049 is a recombinant form of the toxoid of Staphylococcal Enterotoxin B (SEB) with genetic mutations which render the protein non-toxic. NR-10049 contains a hexa-histidine tag near the N-terminus. The recombinant protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-10049 has a theoretical molecular weight of approximately 30 kilodaltons.

The amino acid sequence of SEB from Staphylococcus aureus subsp. aureus, strain COL has been determined (GenPept: AAW37877). The crystal structure of SEB has been solved to 1.48 Å (PDB: 3SEB).

Each vial of NR-10049 contains approximately 1 mg of SEB toxoid in phosphate buffered saline (pH 7.4).

NR-10049 reacts with rabbit polyclonal antibody to SEB using western blot analysis.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Staphylococcal Enterotoxin B Toxoid, Recombinant from Escherichia coli, NR-10049."
Publications Citing
this Reagent:
Turner, K. B., et al. "Isolation and Epitope Mapping of Staphylococcal Enterotoxin B Single-Domain Antibodies." Sensors 14 (2014): 10846-10863. PubMed: 24949641.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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