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NR-14827   Mycobacterium tuberculosis, Strain HN878, Culture Filtrate Proteins
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain HN878, Culture Filtrate Proteins
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14827 is a preparation of culture filtrate proteins (CFP) from Mycobacterium tuberculosis (M. tuberculosis), strain HN878 and contains most of the excreted and secreted proteins of the organism.

The culture was grown to late log phase in glycerol-alanine-salts medium. The culture supernatant was harvested from the live cells and the CFP was concentrated. The concentrated material was dialyzed against 10 mM ammonium bicarbonate and quantitated with the BCA protein assay. Individual lots are subjected to quality control procedures to ensure uniformity and lack of bacterial contamination.

Each vial of NR-14827 contains approximately 1 mg of culture filtrate proteins from M. tuberculosis, strain HN878 in 10 mM ammonium bicarbonate.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain HN878, Culture Filtrate Proteins, NR-14827."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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