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NR-14828   Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14828 is a preparation of the cell wall fraction of Mycobacterium tuberculosis, strain H37Rv and contains proteins and non-protein compounds such as mAGP.

The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 x g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen.

Each vial contains approximately 1 mg of NR-14828 provided as a frozen pellet.

Note: NR-14828 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used. Cell wall fractions may not fully solubilize, but a uniform suspension can be achieved with bath sonification.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Cell Wall Fraction, NR-14828."
Publications Citing
this Reagent:
Gopal, R., et al. "Unexpected Role for IL-17 in Protective Immunity against Hypervirulent Mycobacterium tuberculosis HN878 Infection." PLoS Pathog. 10 (2014): e1004099. PubMed: 24831696.

Kothari, H., et al. "Mycobacterium tuberculosis Infection and Tissue Factor Expression in Macrophages." PLoS One 7 (2012): e45700. PubMed: 23029190.

Matange, N., M. Podobnik, and S. S. Visweswariah. "The Non-Catalytic 'Cap Domain' of a Mycobacterial Metallophosphoesterase Regulates its Expression and Localization in the Cell." J. Biol. Chem. 289 (2014): 22470-22481. PubMed: 24970891.

Nouailles, G., et al. "CXCL5-Secreting Pulmonary Epithelial Cells Drive Destructive Neutrophilic Inflammation in Tuberculosis." J. Clin. Invest. 124 (2014): 1268-1282. PubMed: 24509076.

Perley, C. C., et al. "The Human Antibody Response to the Surface of Mycobacterium tuberculosis." PLoS One 9 (2014): e98938. PubMed: 24918450.

Reyes, F., et al. "Immunogenicity and Cross-Reactivity against Mycobacterium tuberculosis of Proteoliposomes Derived from Mycobacterium bovis BCG." BMC Immunol. 14 (2013): S7. PubMed: 23458692.

Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.

Ryndak, M. B., et al. "Transcriptional Profiling of Mycobacterium tuberculosis Replicating Ex Vivo in Blood from HIV-and HIV+ Subjects." PLoS One 9 (2014): e94939. PubMed: 24755630.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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