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NR-14835  Mycobacterium tuberculosis, CDC1551, Cytosol Fraction
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: CDC1551, Cytosol Fraction
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14835 is a preparation of the cytosol fraction of Mycobacterium tuberculosis, strain CDC1551, and contains cytosolic proteins and soluble material released from the cell wall during disruption of the bacilli.

The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 × g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g. The supernatant was subjected to a 100,000 × g centrifugation for four hours, then collected and dialyzed against 10 mM ammonium bicarbonate. The protein content was determined using the BCA protein assay.

Each vial contains approximately 1 mg of NR-14835 provided in 10 mM ammonium bicarbonate.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Cytosol Fraction, NR-14835."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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