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NR-14837   Mycobacterium tuberculosis, Strain H37Rv, Total Lipids
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain H37Rv, Total Lipids
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14837 is a preparation of the total cellular lipids of irradiated Mycobacterium tuberculosis, strain H37Rv, including those with known biological activities, such as trehalose dimycolate (TDM) and sulpholipids. The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS, inactivated by gamma irradiation and dried. The cellular lipids were extracted with 30 mL of chloroform/methanol (2:1) per gram of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch Wash). The organic phase of the Folch wash was collected and dried.

Each vial contains approximately 5mg of dried total lipids from M. tuberculosis, strain H37Rv.

Note: Total lipid is soluble in chloroform:methanol (2:1). Chloroform or DMSO can also be used depending on the downstream application.

Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Total Lipids, NR-14837.”
Publications Citing
this Reagent:
Gopal, R., et al. "Unexpected Role for IL-17 in Protective Immunity against Hypervirulent Mycobacterium tuberculosis HN878 Infection." PLoS Pathog. 10 (2014): e1004099. PubMed: 24831696.

Kothari, H., et al. "Mycobacterium tuberculosis Infection and Tissue Factor Expression in Macrophages." PLoS One 7 (2012): e45700. PubMed: 23029190.

Reyes, F., et al. "Immunogenicity and Cross-Reactivity against Mycobacterium tuberculosis of Proteoliposomes Derived from Mycobacterium bovis BCG." BMC Immunol. 14 (2013): S7. PubMed: 23458692.

Nouailles, G., et al. "CXCL5-Secreting Pulmonary Epithelial Cells Drive Destructive Neutrophilic Inflammation in Tuberculosis." J. Clin. Invest. 124 (2014): 1268-1282. PubMed: 24509076.

Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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