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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-14841   Mycobacterium tuberculosis, Strain H37Rv, TX-114 Soluble Proteins
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain H37Rv, TX-114 Soluble Proteins
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14841 is a preparation of the TX-114 soluble proteins of Mycobacterium tuberculosis, strain H37Rv.

The culture was grown to late-log phase in glycerol-alanine-salts medium, washed with PBS and inactivated by gamma-irradiation. Cells were broken by French press in 4% triton X-114, partitioned at 37°C and centrifuged. The upper aqueous layer was removed and the process was repeated twice to extract the triton layer. The final triton layer was precipitated in acetone to remove the triton, and the resulting pellet was phenol-extracted, and then dialyzed in water. The protein content of the final product was then quantified using the BCA protein assay.

Each vial contains approximately 1 mg of NR-14841 in water provided as a frozen pellet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv TX-114 Soluble Proteins, NR-14841."
Publications Citing
this Reagent:
Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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