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NR-14843  Mycobacterium tuberculosis, H37Rv, Acetone-Insoluble Lipids
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: H37Rv, Acetone-Insoluble Lipids
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14843 is a preparation of acetone-insoluble cellular lipids extracted from Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv. Total cellular lipids were triturated in acetone at -20°C. The resulting suspension was then centrifuged and the pellet resuspended in chloroform/methanol (2:1) to obtain the acetone-insoluble lipid fraction.

Each vial contains approximately 500 µg of dried acetone-insoluble lipids from M. tuberculosis, strain H37Rv.

Note: Acetone-insoluble lipids are soluble in chloroform:methanol (2:1). Chloroform or DMSO can also be used depending on the downstream application.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Acetone-Insoluble Lipids, NR-14843."
Publications Citing
this Reagent:
Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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