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NR-14848   Mycobacterium tuberculosis, Strain H37Rv, Purified Lipoarabinomannan (LAM)
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain H37Rv, Purified Lipoarabinomannan (LAM)
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14848 is a preparation of lipoarabinomannan (LAM), also referred to as mannosylated lipoarabinomannan (ManLAM), derived from the cell wall of irradiated Mycobacterium tuberculosis, strain H37Rv. LAM possesses many biological activities including immunogenicity, induction of TNF and the release of other cytokines, and inhibition of antigen processing. The nonreducing termini of H37Rv LAM are extensively capped with mannose.

Each vial contains approximately 500 µg of lyophilized purified LAM from Mycobacterium tuberculosis, strain H37Rv. Lyophilized products may aggregate as a loose powder near the lid. Please take precautionary measures (such as tapping the bottom of the tube on the lab bench) to reduce escape of the reagent when opening the vial to reconstitute contents.

Note: LAM can be reconstituted in water. A 100 mM to 500 mM aqueous buffered salt solution, such as phosphate buffered saline, may also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Purified Lipoarabinomannan (LAM), NR-14848."
Publications Citing
this Reagent:
Cavazos, A. "Determining the Role of the Mycobacterium tuberculosis Serine/Threonine Protein Kinase, PknH, in Cell Signaling." (2012).

Hiatt, L. A. and D. E. Cliffel. "Real-Time Recognition of Mycobacterium tuberculosis and Lipoarabinomannan Using the Quartz Crystal Microbalance." Sens. Actuators B: Chem. 174 (2012): 245-252. PubMed: 23175600.

Macdonald, S. H., et al. "Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis." PLoS One 7 (2012): e38488. PubMed: 22675566.

Mazurek, J., et al. "Divergent Effects of Mycobacterial Cell Wall Glycolipids on Maturation and Function of Human Monocyte-Derived Dendritic Cells." PLoS One 7 (2012): e42515. PubMed: 22880012.

Mukundan, H., et al. "Rapid Detection of Mycobacterium tuberculosis Biomarkers in a Sandwich Immunoassay Format Using a Waveguide-Based Optical Biosensor." Tuberculosis 92 (2012): 407-416. PubMed: 22710249.

Mukundan, H., et al. "Understanding the Interaction of Lipoarabinomannan with Membrane Mimetic Architectures." Tuberculosis 92 (2012): 38-47. PubMed: 22033469.

Nouailles, G., et al. "CXCL5-Secreting Pulmonary Epithelial Cells Drive Destructive Neutrophilic Inflammation in Tuberculosis." J. Clin. Invest. 124 (2014): 1268-1282. PubMed: 24509076.

Perley, C. C., et al. "The Human Antibody Response to the Surface of Mycobacterium tuberculosis." PLoS One 9 (2014): e98938. PubMed: 24918450.

Reyes, F., et al. "Immunogenicity and Cross-Reactivity against Mycobacterium tuberculosis of Proteoliposomes Derived from Mycobacterium bovis BCG." BMC Immunol. 14 (2013): S7. PubMed: 23458692.

Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.

Sakamuri, R. M., et al. "Association of Lipoarabinomannan with High Density Lipoprotein in Blood: Implications for Diagnostics." Tuberculosis 93 (2013): 301-307. PubMed: 23507184.

Singhal, J., et al. "Suppression of Dendritic Cell-Mediated Responses by Genes in Calcium and Cysteine Protease Pathways during Mycobacterium tuberculosis Infection." J. Biol. Chem. 287 (2012): 11108-11121. PubMed: 22337888.

Zhang, J. and X. Zhou. "Novel 3-Dimensional Dendrimer Platform for Glycolipid Microarray." Biosens. Bioelectron. 28 (2011): 355-361. PubMed: 21820887.

Ziegenbalg, A., et al. "Immunogenicity of Mycobacterial Vesicles in Humans: Identification of a New Tuberculosis Antibody Biomarker." Tuberculosis 93 (2013): 448-455. PubMed: 23562367.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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