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NR-14854   Mycobacterium tuberculosis, Strain H37Rv, Purified Mycolic Acid Methyl Esters
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain H37Rv, Purified Mycolic Acid Methyl Esters
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature (dry atmosphere)
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14854 contains a preparation of mycolic acid methyl esters (MAME) that were purified from the mycolyl-arabinogalactan peptidoglycan (mAGP) complex of Mycobacterium tuberculosis (M. tuberculosis). mAGP was subjected to alkaline hydrolysis and the mycolic acids were collected and neutralized. Chloroform and water are added to the mycolic acid suspension to form a biphasic partition, and the organic fraction containing mycolic acid is collected and dried.

Each tube contains approximately 1 mg of dried MAME from M. tuberculosis, strain H37Rv.

Note: MAME is soluble in DMSO or chloroform.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Purified Mycolic Acid Methyl Esters, NR-14854."
Publications Citing
this Reagent:
Rodriguez, M. E., et al. "Mycobacterial Phosphatidylinositol Mannoside 6 (PIM6) Up-Regulates TCR-Triggered HIV-1 Replication in CD4+ T Cells." PLoS One 8 (2013): e80938. PubMed: 24282561.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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