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NR-19328   Mycobacterium leprae, Cytosol Fraction (MLSA) minus LAM
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Mycobacterium leprae, Cytosol Fraction (MLSA) minus LAM
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19328 was produced from purified Mycobacterium leprae (M. leprae) whole cells (non-irradiated, non-autoclaved) extracted from a pool of infected armadillo liver and spleen tissue. Cells were sonicated for forty, one-minute cycles and an acid fast stain of the sonicate was performed to confirm complete breakage of the cells. The sonicate was centrifuged to pellet the cell wall material and the resulting supernatant was further centrifuged to pellet the cell membrane material. The LAM was removed from the cytosolic protein fraction by TX-114 extraction. The final supernatant was collected and the protein content quantitated by BCA assay.

Note: In addition to cytosolic proteins this preparation will contain soluble material released from the cell wall during the disruption process.

Each vial contains approximately 250 µg of lyophilized MLSA minus LAM pooled from up to three different strains of M. leprae. Please refer to the Certificate of Analysis for information regarding the specific strains used in the production of each lot.

Note: MLSA minus LAM can be reconstituted in sterile phosphate buffered saline, pH 7.2, or another suitable buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae Cytosol Fraction (MLSA) minus LAM, NR-19328."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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