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NR-19331   Mycobacterium leprae, Cell Membrane Fraction (MLMA)
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Mycobacterium leprae, Cell Membrane Fraction (MLMA)
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -80°C or colder
Contributor: BEI Resources or NIH - Leprosy Research Support Contract
Comments:
NR-19331, Cell Membrane Fraction (MLMA) from Mycobacterium leprae, is no longer available.

NR-19331 was produced from purified Mycobacterium leprae (M. leprae) whole cells (non-irradiated, non-autoclaved) extracted from a pool of infected armadillo liver and spleen tissue. Cells were sonicated for forty, one-minute cycles and an acid fast stain of the sonicate was performed to confirm complete breakage of the cells. The sonicate was centrifuged to pellet the cell wall material and the resulting supernatent was further centrifuged to pellet the cell membrane material. The cell membrane pellet was washed with phosphate buffered saline and the washed membrane preparation was quantitated for protein content by BCA assay.

Note: This preparation contains cytoplasmic membrane and components of the outer membrane.

Each vial contains approximately 250 µg of lyophilized MLMA pooled from up to three different strains of M. leprae. Please refer to the Certificate of Analysis for information regarding the specific strains used in the production of each lot.

Note: MLMA can be reconstituted in distilled water, sterile phosphate buffered saline, pH 7.2, or another suitable buffer.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae Cell Membrane Fraction (MLMA), NR-19331."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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