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NR-19332   Mycobacterium leprae, Total Lipids
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Mycobacterium leprae, Total Lipids
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature
Contributor: BEI Resources or NIH - Leprosy Research Support Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19332 is a preparation of the total cellular lipids of Mycobacterium leprae, including those with known biological activities, including phenolic glycolipid I (PGL-I), II and III and dimycocerosate (DIM). The cellular lipids were extracted from a pool of armadillo-derived purified liver and spleen whole cells with 5 mL of chloroform/methanol (2:1) per 200 mg of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch wash). The organic phase of the Folch wash was collected and dried.

Each vial contains approximately 500 µg of dried total lipids pooled from up to three different strains of M. leprae. Please refer to the Certificate of Analysis for information regarding the specific strains used in the production of each lot.

Note: Total lipid is soluble in chloroform:methanol (2:1). Chloroform or DMSO can also be used depending on the downstream application.
Citations: Acknowledgment for publications should read "The following reagent was obtained through the BEI Resources, NIAID, NIH: Mycobacterium leprae Total Lipids, NR-19332."
PERMITS
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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