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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-19332  Mycobacterium leprae, Total Lipids
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Total Lipids
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature
Contributor: BEI Resources or NIH - Leprosy Research Support Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19332 is a preparation of the total cellular lipids of Mycobacterium leprae (M. leprae), including those with known biological activities, including phenolic glycolipid I (PGL-I), II and III and dimycocerosate (DIM). The cellular lipids were extracted from a pool of armadillo-derived purified liver and spleen whole cells with 5 mL of chloroform/methanol (2:1) per 200 mg of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch wash). The organic phase of the Folch wash was collected and dried.

Each vial contains approximately 500 µg of dried total lipids pooled from up to three different strains of M. leprae.

Note: Total lipid is soluble in chloroform/methanol (2:1). Chloroform or DMSO can also be used depending on the downstream application.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae Total Lipids, NR-19332."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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