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NR-19345   Mycobacterium leprae, Monodeglycosylated PGL-I
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Mycobacterium leprae, Monodeglycosylated PGL-I
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: Room temperature
Contributor: NIH - Leprosy Research Support Contract
Comments:
NR-19345, Mycobacterium leprae Monodeglycosylated PGL-I, is no longer available.

NR-19345 was produced by acid hydrolysis using 0.3 N HCl at 100°C for two hours, which leads to formation of multiple products and some unhydrolyzed PGL-I. Preparative TLC in ether:acetone (9:1, v/v) with scraping of individual bands and elution of specific components from silica gives monodeglycosylated PGL-I.

Each vial contains approximately 250 µg of dried monodyglycosolated PGL-I pooled from up to three different strains of M. leprae. Please refer to the Certificate of Analysis for information regarding the specific strains used in the production of each lot.

Note: Monodyglycosolated PGL-I is soluble in 2:1 chloroform methanol or 100% methanol or ethanol. NR-19345 is not soluble in aqueous buffers.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae Monodeglycosylated PGL-I, NR-19345."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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