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NR-36491   Mycobacterium tuberculosis, Strain Indo-Oceanic T17X, Gamma-Irradiated Whole Cells
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain Indo-Oceanic T17X, Gamma-Irradiated Whole Cells
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: K Dobos
Comments:
Quantity limit for this item is 2 per year. Orders over this limit will be sent to NIAID for approval before shipment.

Mycobacterium tuberculosis (M. tuberculosis), strain Indo-Oceanic T17X was grown to late-log phase in glycerol-alanine-salts medium and inactivated by exposure to 2.4 mRads of ionizing gamma irradiation using a 137Cs source. Confirmation of inactivation was performed by Alamar Blue assay. A dose of 2.4 mRads of gamma irradiation kills M. tuberculosis to a 1020 degree of certainty while maintaining 93% to 95% of the biological activity of the enzymes. The bacilli are harvested by filtration and washed with PBS pH 7.4.

Each vial contains approximately 10 g of NR-36491 provided as a cell culture pellet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain Indo-Oceanic T17X, Gamma-Irradiated Whole Cells, NR-36491."
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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