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NR-36499  Mycobacterium tuberculosis, Indo-Oceanic T17X, Cell Wall Fraction
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Indo-Oceanic T17X, Cell Wall Fraction
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-36499 is a preparation of the cell wall fraction of Mycobacterium tuberculosis, strain Indo-Oceanic T17X, and contains proteins and non-protein compounds such as mAGP.

The culture was grown to late log phase in glycerol-alanine-salts medium, washed with PBS pH 7.4, and inactivated by gamma irradiation. The bacilli were suspended at a concentration of 2 g/mL in PBS containing 8 mM EDTA, DNase, RNase, and a proteinase inhibitor tablet, and broken in a French Press pressure cell at 4°C. Unbroken cells were removed by low speed (3,000 x g) centrifugation. The cell wall was isolated by centrifugation at 27,000 × g for one hour and washed two times in PBS. The final cell wall pellet was suspended and dialyzed in 10 mM ammonium bicarbonate, quantified by BCA protein assay for protein content, and frozen.

Each vial contains approximately 1 mg of protein in 10 mM ammonium bicarbonate provided as a frozen pellet.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain Indo-Oceanic T17X, Cell Wall Fraction, NR-36499."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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