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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-44090   Lipopolysaccharide from a Non-Toxigenic Mutant of Escherichia coli, Strain 86-24 (Serotype O157:H7)
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Description: Lipopolysaccharide from a Non-Toxigenic Mutant of Escherichia coli, Strain 86-24 (Serotype O157:H7)
Biosafety Level: 1
Availability Status: In Stock
Store at: 4°C or colder
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Escherichia coli (E. coli), strain 86-24, serotype O157:H7, is a toxigenic isolate that was discovered in 1986 during an outbreak in Walla Walla, Washington, USA. It is an enterohemorrhagic E. coli (EHEC) strain, known to produce shiga-like toxin 2. Lipopolysaccharide (LPS) was isolated from a non-toxigenic mutant of strain 86-24 using phenol/chloroform extraction. The extract was dialyzed against water, to remove the phenol, and dehydrated.

Each vial of NR-44090 contains approximately 1 µg of dehydrated LPS extracted from a non-toxigenic mutant of E. coli, strain 86-24. It is recommended that the material be rehydrated with 1 mL of distilled water.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Lipopolysaccharide from a Non-Toxigenic Mutant of Escherichia coli, Strain 86-24 (Serotype O157:H7), NR-44090."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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