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NR-44099  Mycobacterium tuberculosis, Indo-Oceanic T17X, Total Lipids
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Indo-Oceanic T17X, Total Lipids
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature (dry atmosphere)
Contributor: BEI Resources
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-44099 is a preparation of the total cellular lipids of irradiated Mycobacterium tuberculosis (M. tuberculosis), strain Indo-Oceanic T17X, including those with known biological activities, such as trehalose dimycolate (TDM) and sulpholipids. The culture was grown to latelog phase in glycerol-alanine-salts medium, washed with PBS, inactivated by gamma irradiation and dried. The cellular lipids were extracted with 30 mL of chloroform:methanol (2:1) per gram of cells at 55°C for 18 hours. Cells were removed by filtration and contaminating hydrophilic molecules were removed by biphasic partitioning with water (Folch Wash). The organic phase of the Folch wash was collected and dried.

Each vial contains approximately 5 mg of dried total lipids from M. tuberculosis, strain Indo-Oceanic T17X.

Note: Total lipid is soluble in chloroform:methanol (2:1). DMSO can also be used depending on the downstream application.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain Indo-Oceanic T17X, Total Lipids, NR-44099."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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