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NR-49461   Zaire Ebolavirus, Guéckédou/Guinea C07, Gamma-Irradiated
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Zaire ebolavirus
Description: Zaire Ebolavirus, Guéckédou/Guinea C07, Gamma-Irradiated
Biosafety Level: 1
Availability Status: In Stock
Store at: -70°C or colder
Contributor: World Reference Center for Emerging Viruses and Arboviruses
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Gamma-irradiated Zaire ebolavirus, Guéckédou/Guinea C07 was prepared from infected Vero E6 cell pellets. Cell pellets were resuspended in 50 mM sodium borate and 120 mM sodium chloride (pH 9) containing 1% Triton X-100, gamma-irradiated (5 × 10RADs) on dry ice, and sonicated. Cell debris was removed by centrifugation and the supernatant containing the irradiated antigen was aliquoted.

NR-49461 was tested for residual virus following the procedure described by Towner et al. No residual virus was recovered.

Each vial contains 100 µL of irradiated antigen 50 mM sodium borate and 120 mM sodium chloride (pH 9) containing 1% Triton X-100.  The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Zaire Ebolavirus, Guéckédou/Guinea C07, Gamma-Irradiated, NR-49461.”
For a list of permits that may be required for shipping this product and to set the permit information preferences; please select a country from the drop down below.

  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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Vero E6, Uninfected Cell Lysate, Gamma-Irradiated Cercopithecus aethiops
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