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BEI Resources currently limits the total number of "Made to Order" items which can be ordered by a registrant to 10 "Made to Order" items per 6 month period. Please check the Availability Status on the items you are ordering and limit orders appropriately.
NR-49815  Mesocricetus auratus, BHK-21, Uninfected Cell Lysate, Gamma-Irradiated
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mesocricetus auratus
Description: BHK-21, Uninfected Cell Lysate, Gamma-Irradiated
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: World Reference Center for Emerging Viruses and Arboviruses
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Uninfected BHK-21 cell pellets were resuspended in 50 mM sodium borate and 120 mM sodium chloride (pH 9) containing 1% Triton X-100, gamma-irradiated (5 × 106 RADs) on dry ice and sonicated. Cell debris was removed by centrifugation and the supernatant containing the irradiated antigen was aliquoted and vialed. NR-49815 is intended for use as a control with gamma-irradiated viral antigens prepared from infected BHK-21 cells.

Each vial contains 100 µL of irradiated antigen in 50 mM sodium borate and 120 mM sodium chloride (pH 9) containing 1% Triton X-100. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: BHK-21, Uninfected Cell Lysate, Gamma-Irradiated, NR-49815.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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