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NR-50369  Zika virus, PRVABC59, Heat-Inactivated
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Zika virus, PRVABC59, Heat-Inactivated
Description: Zika virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: BJ Russell
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

ZIKV, PRVABC59, heat inactivated is a preparation of Zika virus (ZIKV), PRVABC59 that has been inactivated by heating to 65°C for 30 minutes.

ZIKV, PRVABC59 was isolated from the blood of a human in Puerto Rico in December 2015. The complete genomic sequence of the ZIKV, PRVABC59 serum isolate was previously determined (GenBank: KU501215). The complete coding sequence of BEI Resources ZIKV, PRVABC59 (NR-50240 lot 64112564) has also been determined (GenBank: KX087101). The E protein of the tissue culture grown ZIKV PRVABC59 isolate (GenBank: KX087101) has a valine to leucine mutation at position 330 relative to the original patient serum ZIKV PRVABC59 isolate (GenBank: KU501215). The ZIKV, PRVABC59 E-V330L mutant is shown to be less pathogenic in mice, with delayed mortality and decreased viral dissemination to eye and brain as compared to the original ZIKV PRVABC59 serum isolate.

This product is intended for use as a positive control in molecular assays for the qualitative detection and identification of ZIKV. NR-50369 has not been tested in other applications. It does not contain intact ZIKV genomic RNA, and should not be used as a source of nucleic acid for transfection or for RT-PCR of “gene-length” amplicons.

Each vial contains approximately 0.25 mL of heat-inactivated, clarified cell lysate and supernatant from Cercopithecus aethiops kidney epithelial cells (Vero E6, ATCC ® CRL-1586™) infected with ZIKV, PRVABC59.
Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Zika Virus, PRVABC59, Heat-Inactivated, NR-50369.”
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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