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  Question How much DNA/RNA is there in a worm (From FR3 Collection)?
Author: BEI Resources
Submitted on: 9/4/2013 3:44:48 PM
Average rating:
 
  Answer

Using FR3 protocols, we estimate the following:

Total RNA:

  • 500 L3s yields 2.5-5 ug RNA => 5-10 ng RNA/L3
  • 500 L4s yields 15-20 ug RNA => 30-40 ng RNA/L4
  • 1 million mf yield 22 ug RNA => 2 ng RNA/100 mf
  • 160 immature females yield 65 ug RNA => 400 ng RNA/female
  •   8 adult males yield 14 ug RNA => 1.8 ug RNA/male

DNA:

  • 500 L3s yield ~3 ug DNA => 6 ng DNA/L3
  • 500 L4s yield ~6 ug DNA => 12 ng DNA/L4
  • 5 adult females yield ~5 ug DNA => 1 ug DNA/adult female
 
Last Updated: 9/4/2013 3:44:48 PM
 

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