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NR-48696   Mycobacterium tuberculosis, Strain H37Rv, Gamma-Irradiated Whole Cells Kit Containing Hypoxic and Normoxic Cultures
(Assays/Panels)
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Description: Mycobacterium tuberculosis, Strain H37Rv, Gamma-Irradiated Whole Cells Kit Containing Hypoxic and Normoxic Cultures
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources
Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.


NR-48696 is a kit comprised of gamma-irradiated cells from two preparations of Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv.

M. tuberculosis, strain H37Rv hypoxic culture was grown to early log phase in glycerol-alanine-salts medium, and the cells were sparged with nitrogen gas to slowly deplete oxygen. The cells were grown an additional period of time and then harvested.

M. tuberculosis, strain H37Rv normoxic culture was grown to mid log phase in glycerol-alanine-salts medium, and the cells were sparged with air and harvested.

Following harvest, both cultures were inactivated by exposure to a Cs source with delivery of 1.44 to 2.4 MRad of ionization radiation (dose range is provided due to variation across the sample chamber).


Each kit consists of one vial of irradiated whole cells from a hypoxic culture and one vial of irradiated whole cells from a normoxic culture of M. tuberculosis, strain H37Rv, each containing approximately 1 g of cells provided as a cell culture pellet.

Citations: Acknowledgment for publications should read “The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain H37Rv, Gamma-Irradiated Whole Cells Kit Containing Hypoxic and Normoxic Cultures, NR-48696.”
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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