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NR-2638   Genomic DNA from Vaccinia Virus, New York City Board of Health (Wyeth, Calf adapted)
(Nucleic Acids)
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at no cost to registered researchers.
Description: Genomic DNA from Vaccinia Virus, New York City Board of Health (Wyeth, Calf adapted)
Organism: Vaccinia virus
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: ATCC®
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Genomic DNA was isolated from a preparation of cell lysate and supernatant from cells infected with vaccinia virus, IHD (BEI Resources NR-54).

Lot 5075334 was prepared from infected Cercopithecus aethiops kidney epithelial cells (MA-104 Clone 1; ATCC ® CRL-2378.1™). Lot 63421353 was prepared from infected Cercopithecus aethiops kidney epithelial cells (Vero; ATCC ® CCL-81™).

The NYCBH (Wyeth, calf adapted) strain was derived from a strain obtained by the NYCBH by multiple passage in calf skin. It was used to develop Dryvax®, the live-virus vaccine used during both the World Health Organization vaccination program ending in the mid 1970s and the vaccination programs of the Department of Health and Human Services and Department of Defense which began in 2002.

NR-2638 has been qualified for PCR applications by amplification of either an approximately 2600 bp sequence or an approximately 1100 bp sequence. NR-2638 is not intended for use as a standard for quantitative PCR.

Each vial of lot 5075334 contains a target amount of 1 ×108 copies of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.5) containing sodium azide. The actual number of copies per vial may vary from 10-fold lower to 10-fold higher.

Each vial of lot 63421353 contains 100 µL of viral genomic DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 7.0).

The viral genomic DNA is in a background of cellular nucleic acid and carrier RNA. The vial should be centrifuged prior to opening.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Genomic DNA from Vaccinia Virus, New York City Board of Health (Wyeth, Calf adapted), NR-2638."
Publications Citing
this Reagent:
Baker, J. L., and B. M. Ward. "Development and Comparison of a Quantitative TaqMan-MGB Real-Time PCR Assay to Three Other Methods of Quantifying Vaccinia Virions." J. Virol. Methods 196 (2014): 126-132. PubMed: 24211297.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-54
Vaccinia virus New York City Board of Health (Wyeth, Calf Adapted)
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