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NR-36431   Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis
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Description: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -80°C or colder
Contributor: NIH - TB Vaccine Testing and Research Materials Contract
NR-36431 is no longer available. Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis, is now available as NR-50170.

NR-36431 is a recombinant expression vector containing Mycobacterium tuberculosis gene Rv3875, which encodes the early secretory antigenic target Esat6, also known as esxA. Gene Rv3875 was amplified by PCR and cloned into pET23b for expression in Escherichia coli. The gene was cloned without a signal sequence. The nucleotides for the amino acids phenylalanine, alanine, leucine and glutamic acid (FALE) were added to the gene just prior to the histidine tag to increase plasmid stability and promote solubility upon transformation and expression. The expressed protein has an observed molecular weight of 10 kDa. The expected purified protein yield from a one liter culture is approximately 5 mg.

Note: Plasmid pMRLB7 contains the gene required for ampicillin (Ap) resistance. The recommended concentration of Ap in culture is 100 µg/mL.

Each vial contains approximately 1 µg of plasmid DNA in TE buffer (10 mM Tris-HCl, 1 mM EDTA buffer, pH 8).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Plasmid pMRLB.7 Containing Gene Rv3875 (Protein Esat6) from Mycobacterium tuberculosis, NR-36431."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

Plasmid pMRLB.7 Containing Gene Rv3875 (Protein ESAT-6) from Mycobacterium tuberculosis
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