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NR-14855   Ag85 Complex, Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
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Description: Ag85 Complex, Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

The Antigen 85 Complex was isolated from Mycobacterium tuberculosis (M. tuberculosis), strain H37Rv culture filtrate proteins and purified. This complex of fibronectin-binding proteins Ag85A (FbpA), Ag85B (FbpB), and Ag85C (FbpC) is the major secreted protein component of M. tuberculosis culture fluids and plays a key role in the pathogenesis of tuberculosis. This protein complex is highly immunogenic and plays a role in cell wall assembly via a mycolyltransferase exchange process. All three purified antigens have shown extensive cross-reactivity and stimulate complement-mediated phagocytosis by host macrophages.

Each vial contains approximately 500 µg of lyophilized, purified Ag85 complex from Mycobacterium tuberculosis, strain H37Rv in 10 mM ammonium bicarbonate.

Note: NR-14855 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Ag85 Complex, Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-14855."
Publications Citing
this Reagent:
Cyktor, J. C., et al. "IL-10 Inhibits Mature Fibrotic Granuloma Formation during Mycobacterium tuberculosis Infection." J. Immunol. 190 (2013): 2778-2790. PubMed: 23396944.

Dhiman, R., et al. "NK1.1+ cells and IL-22 Regulate Vaccine-Induced Protective Immunity against Challenge with Mycobacterium tuberculosis." J. Immunol. 189 (2012): 897-905. PubMed: 22711885.

Ferrara, F., et al. "Using Phage and Yeast Display to Select Hundreds of Monoclonal Antibodies: Application to Antigen 85, A Tuberculosis Biomarker." PLoS One 7 (2012): e49535. PubMed: 23166701.

Horvath, C., et al. "Mechanisms of Delayed Anti-Tuberculosis Protection in the Lung of Parenteral BCG-Vaccinated Hosts: A Critical Role of Airway Luminal T Cells." Mucosal Immunol. 5 (2012): 420-431. PubMed: 22453678.

Jeyanathan, M., et al. "Differentially Imprinted Innate Immunity by Mucosal Boost Vaccination Determines Antituberculosis Immune Protective Outcomes, Independent of T-cell Immunity." Mucosal Immunol. 6 (2012): 612-625. PubMed: 23131783.

Khan, M. K., et al. "In silico Predicted Mycobacterial Epitope Elicits In Vitro T-Cell Responses." Mol. Immunol. 61 (2014): 16-22. PubMed: 24853589.

Macdonald, S. H., et al. "Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis." PLoS One 7 (2012): e38488. PubMed: 22675566.

Mukundan, H., et al. "Rapid Detection of Mycobacterium tuberculosis Biomarkers in a Sandwich Immunoassay Format Using a Waveguide-Based Optical Biosensor." Tuberculosis 92 (2012): 407-416. PubMed: 22710249.Ziegenbalg, A., et al. "Immunogenicity of Mycobacterial Vesicles in Humans: Identification of a New Tuberculosis Antibody Biomarker." Tuberculosis 93 (2013): 448-455. PubMed: 23562367.
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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