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NR-15771   Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli
(Proteins)
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at no cost to registered researchers.
Description: Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli
Organism: Yersinia pestis
Biosafety Level: 1
Availability Status: Temporarily Out of Stock (Please See Comments)
Store at: -80°C or colder
Contributor: BEI Resources
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

This item is currently in our production queue. Please allow ample time for distribution lots to be made available.


NR-15771 is a recombinant form of the Yersinia pestis (Y. pestis) fraction 1 capsular antigen (F1). F1 is a plasmid (pFra)-encoded proteinaceous capsule synthesized in large quantities by Y. pestis and reported to confer antiphagocytic properties on the pathogen by interfering with complement-mediated opsonization. The protein is highly immunogenic and has been indirectly associated with eliciting a protective immune response in humans. The recombinant protein lacks the native signal sequence but includes an N-terminal hexa-histidine tag, a thrombin cleavage site, and amino acid residues 22 to 170 of F1 from Y. pestis (GenPept: AAS58714). The protein was expressed in Escherichia coli and purified by nickel affinity chromatography. NR-15771 has a theoretical molecular weight of 17,858 daltons.

Each vial contains approximately 300 µg of NR-15771 in PBS.

NR-15771 reacts with monoclonal antibody to Y. pestis F1 antigen in ELISA and Western blot assays.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Fraction 1 Capsular Antigen (F1) from Yersinia pestis with N-terminal Histidine Tag, Expressed in Escherichia coli, NR-15771."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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