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NR-50350   Escherichia coli, DH5αλpir
(Bacteria)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Designations: DH5αλpir
Biosafety Level: 1
Availability Status: In Stock
Store at: -60°C or colder
Contributor: JL Bose, EV Stabb
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Escherichia coli (E. coli), strain DH5αλpir contains the pir genes which allow genetic manipulations of vectors prior to transfer into Staphylococcus species. Strain DH5αλpir has geneotype F-ϕdlacZ ΔM15 ΔlacZYA-argF U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1.

E. coli strains DH5αλpir and GM2163λpir were deposited in conjunction with vectors pKK22 with pKK30 (available as a kit, BEI Resources NR-50352). pKK22 and pKK30 were created to maintain stability in E. coli and Staphylococcus species without antibiotic selection during in vitro and in vivo experiments. The E. coli R6Kγ origin of replication of both vectors requires pir+ for replication which is provided in either DH5αλpir or GM2163λpir E. coli strains.

Catalog Number
Vector or Host
Comments
NR-50348 pKK22 For use in E. coli DH5αλpir or GM2163λpir or S. sureus USA300 strains containing LAC-p01
NR-50349 pKK30 pKK30 is a variant of pKK22, for use in E. coli DH5αλpir or GM2163λpir or Staphylococcus species not containing LAC-p01
NR-50350 E. coli DH5αλpir Host strain containing the pir genes for performing genetic manipulations prior to transfer into Staphylococcus (F-ϕdlacZ ΔM15 ΔacZYA-argF U169 deoR supE44 hsdR17 recA1 endA1 gyrA96 thi-1 relA1)
NR-50351 E. coli GM2163λpir Host strain containing the pir genes for performing genetic manipulations. This strain is also a dam and dcm methylase mutant for transfer of plasmids into Staphylococcus isolates that do not accept E. coli DNA easily (F-ara-14 leuB6 fhuA31 lacY1 tsx78 glnV44 galK2 galT22 mcrA dcm-6 hisG4 rfbD1 rpsL136 dam13::Tn9 xylA5 mtl-1 thi-1 mcrB1 hsdR2 λpir)

This strain was developed by Krute, et al., at the Department of Microbiology, Molecular Genetics and Immunology, University of Kansas Medical Center, PubMed:  27637878.

Each vial of NR-50350 contains approximately 0.5 mL of E. coli, strain DH5αλpir in Tryptic Soy broth supplemented with 10% glycerol.

Citations: Acknowledgment for publications should read “The following reagent was contributed by Dr. J. L. Bose for distribution by BEI Resources, NIAID, NIH: Escherichia coli, Strain DH5αλpir, NR-50350.”
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

RELATED PRODUCTS
NR-50348
Escherichia coli - Staphylococcus aureus Shuttle Vector pKK22, Recombinant in Escherichia coli
NR-50349
Escherichia coli - Staphylococcus aureus Shuttle Vector pKK30, Recombinant in Escherichia coli
NR-50351
Escherichia coli GM2163λpir
NR-50352
Escherichia coli Escherichia coli - Staphylococcal Shuttle Vectors and Hosts
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