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NR-19342   Mycobacterium leprae, Phenolic Glycolipid-I (PGL-I)
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium leprae
Description: Mycobacterium leprae, Phenolic Glycolipid-I (PGL-I)
Biosafety Level: 1
Availability Status: In Stock
Store at: Room temperature
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-19342 is a preparation of the phenolic glycolipid-1 (PGL-I) of Mycobacterium leprae (M. leprae) extracted from infected armadillo liver and spleen tissue. Crude lipids were obtained from chloroform:methanol (2:1) extraction of tissues and eluted on a silica gel column followed by preparative thin layer chromatography. The fractions containing PGL-I were pooled in chloroform:methanol (2:1) and dried under a nitrogen bath.

Each vial contains approximately 1 mg of dried PGL-1 from M. leprae

Note: PGL-1 can be reconstituted in methanol or ethanol.

Note: PGL-I has a glassy appearance and can be difficult to observe in the glass vial.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium leprae Phenolic Glycolipid-I (PGL-I), NR-19342."
Publications Citing
this Reagent:
Medeiros, M. F., et al. "An Attempt to Improve Pure Neural Leprosy Diagnosis Using Immunohistochemistry Tests in Peripheral Nerve Biopsy Specimens." Appl. Immunohistochem. Mol. Morphol. 22 (2014): 222-230. PubMed: 23702646.

Sakamuri, R. M., et al. "Detection of Stealthy Small Amphiphilic Biomarkers." J. Microbiol. Methods 103 (2014): 112-117. PubMed: 24880131.

Spencer, J. S., and P. J. Brennan. "The Role of Mycobacterium leprae Phenolic Glycolipid I (PGL-I) in Serodiagnosis and in the Pathogenesis of Leprosy." Lepr. Rev. 82 (2011): 344. PubMed: 22439275.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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