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NR-4676   Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Description: Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli
Biosafety Level: 1
Availability Status: In Stock
Store at: -20°C or colder
Contributor: AD O'Brien, JF Sinclair
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-4676 is a recombinant toxoid of Shiga toxin type 2 (Stx2) with genetic mutations in the catalytic A subunit which render the protein non-toxic. The recombinant B subunit includes a C-terminal hexa-histidine tag. The recombinant toxoid was expressed in E. coli and purified by nickel affinity chromatography. NR-4676 has a theoretical molecular weight of approximately 33080 daltons for subunit A and 8640 daltons for subunit B. The predicted amino acid sequence of NR-4676 is shown in Table 1 (Subunit A) and Table 2 (Subunit B) on the product sheet.

The sequences of the structural genes for Shiga toxin from Shigella dysenteriae and Shiga toxin type 2 from Escherichia coli (E. coli) have been determined. The crystal structure of Shiga toxin from Shigella dysenteriae and Shiga toxin type 2 from E. coli have been solved (PDB: 1DM0 and 1R4P, respectively).

Each vial of NR-4676 contains approximately 50 µg of recombinant Stx2 toxoid suspended in phosphate buffered saline.

NR-4676 reacts with rabbit polyclonal antibody to Stx2 and is not cytotoxic in Vero cells.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 2 Toxoid, Recombinant from Escherichia coli, NR-4676."
Publications Citing
this Reagent:
Lee, M. S., et al. "Shiga Toxins Induce Autophagy Leading to Differential Signalling Pathways in Toxin-Sensitive and Toxin-Resistant Human Cells." Cell. Microbiol. 13(2011): 1479-1496. PubMed: 21722286.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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