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NR-858   Shiga Toxin Type 1 Toxoid, Recombinant from Escherichia coli
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Escherichia coli
Description: Shiga Toxin Type 1 Toxoid, Recombinant from Escherichia coli
Biosafety Level: 1
Availability Status: Discontinued (Please See Comments)
Store at: -70°C or colder (Note: The label for lot 57680169 incorrectly indicates that it should be stored at -20°C or colder.)
Contributor: AD O'Brien, JF Sinclair
NR-858, Shiga Toxin Type 1 Toxoid, Recombinant from Escherichia coli, is no longer available. 

NR-858 is a recombinant toxoid of Shiga toxin type 1 (Stx1) with genetic mutations in the catalytic A subunit which render the protein non-toxic. The recombinant A subunit includes an N-terminal hexa-histidine tag. The recombinant B subunit contains a C-terminal hexa-histidine tag. The recombinant protein was expressed in Escherichia coli (E. coli) and purified by nickel affinity chromatography. NR-858 has a theoretical molecular weight of approximately 33534 daltons for subunit A and 8513 daltons for subunit B.

The sequences of the structural genes for Shiga toxin from Shigella dysenteriae and Stx1 from E. coli have been determined. The crystal structure of Shiga toxin from Shigella dysenteriae has been solved (PDB: 1DM0).

NR-858 reacts with rabbit polyclonal antibody to Stx1 and is not toxic to Vero cells.

Each vial of NR-858 contains approximately 50 µg of recombinant Stx1 toxoid suspended in phosphate buffered saline (pH 7.4).
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Shiga Toxin Type 1 Toxoid, Recombinant from Escherichia coli, NR-858."
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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