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The NIH HIV Reagent Program has been transitioned to BEI Resources which is managed by American Type Culture Collection (ATCC). We want to ensure your continued use of the materials within BEI Resources with minimal impact to you. BEI Resources materials are provided at no cost to registered researchers (shipping and handling fee may apply).
This panel of full-length functional gp160 expression vector clones from 56 international HIV-1 primary isolates representing six clades (A, B, C, D, AE, and AG) is a great tool for vaccine and functional immune response studies, which can be used to generate pseudoviruses and naturalization assays. These clones are available in the NIH HIV Reagent Program catalog individually (ARP-13489 to ARP-13523) or as part of a panel set (ARP-13467).
J-Lat cells were generated by infecting Jurkat cells with an HIV-1 pseudotyped virus, where Nef was replaced with GFP, and clones were characterized (ARP-9846 to ARP-9850). Similar latent cells were also established with a simpler retroviral vector, Tat-IRES-EGFP, under the control of LTR, and clones were characterized (ARP-9851 to ARP-9856). All clones can be activated by most Latency Reversing Agents. For analysis, GFP can be measured by flow cytometry or p24 ELISA of culture supernatants.
Peptide pool, HIV-1 subtype B Env (ARP-12540), Gag (ARP-12425), Nef (ARP-12545), Pol (ARP-12438), Vpr (ARP-12860) and Rev (ARP-6445) are of the most requested reagents in the HRP catalog. These peptides are produced as 15-mers with 11 aa overlaps so that T-cell stimulation is optimized while the chance of missing T-cell epitopes is minimized. Peptide arrays for the same HIV-1 viral proteins are also available (ARP-9480, ARP-8117, ARP-5189, ARP-6208, ARP-6447).
Additional SIV and SHIV stocks produced for NIAID are available through Quality Biological.
For information and forms regarding registration with BEI Resources, click here.
After your registration application has been approved by NIAID and ATCC, you will receive a login name and password. After logging in, find the reagent you need using the search function. Select the items from the search results to see the product detail pages. After you read the product detail page description, click the "Add to cart" button to place the item into your shopping cart. The BEI Resources Program materials to include HIV reagents, are provided at no cost to registered researchers (shipping charges may apply). For additional information, click here.
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