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NR-14862   MPT32/Apa (Gene Rv1860), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
(Proteins)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Description: MPT32/Apa (Gene Rv1860), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv
Organism: Mycobacterium tuberculosis
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: BEI Resources or NIH - TB Vaccine Testing and Research Materials Contract
Comments:
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

NR-14862 is a preparation of native MPT32/Apa (Gene Rv1860) purified from sterile-filtered Mycobacterium tuberculosis, strain H37Rv culture filtrate protein fraction. The culture filtrate protein fraction was precipitated with 40% ammonium sulfate, applied to a ConA column, eluted, concentrated, dialyzed, applied to a phenyl sepharose column and eluted. The purified MPT32/Apa was dialyzed in 10 mM ammonium bicarbonate.

Note: MPT32/Apa appears as a doublet by SDS-PAGE. The major component is 45 kDa and the minor component is a 42 kDa degradation product. The mature protein has a calculated molecular weight of 30.2 kDa, however the abundance of proline residues along with glycosylation causes this molecule to migrate at 45 kDa by SDS-PAGE.

Each vial contains approximately 500 µg of lyophilized MPT32/Apa in 10 mM ammonium bicarbonate.

Note: NR-14862 is soluble in 100 mM to 500 mM aqueous buffered salt solutions, such as phosphate buffered saline. A 10 mM ammonium bicarbonate solution can also be used.
Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: MPT32/Apa (Gene Rv1860), Purified Native Protein from Mycobacterium tuberculosis, Strain H37Rv, NR-14862."
Publications Citing
this Reagent:
Macdonald, S. H., et al. "Networked T Cell Death following Macrophage Infection by Mycobacterium tuberculosis." PLoS One 7 (2012): e38488. PubMed: 22675566.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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