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Product Name:
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83972 (Serotype ON:KN)
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Manufacturer:
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BEI Resources
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Taxonomy:
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Bacteria Classification: Enterobacteriaceae, Escherichia
Species: Escherichia coli
Strain: 83972 (also referred to as ABU 83972)1
Serotype: ON:KN (N = nontypeable)1
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Additional Information:
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E. coli is a Gram-negative, rod-shaped bacterium which occurs singly or in pairs. It is a major facultative inhabitant of the large intestine. E. coli, strain 83972 has been shown clinically and experimentally to persist in the human bladder for extended periods without producing overt symptoms of infection, even showing potential as a probiotic.4,5,6 It lacks functional forms of the three uropathogenic E. coli (UPEC) class fimbriae (P, type 1, and F1C) associated with symptomatic urinary tract infections.4,7 E. coli, strain 83972 persists in the human bladder by virtue of fast growth and a failure to trigger host defense mechanisms.4
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Material Provided:
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Each vial contains approximately 0.5 mL of bacterial culture in Tryptic Soy broth supplemented with 10% glycerol.
Note: If homogeneity is required for your intended use, please purify prior to initiating work.
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Packing/Storage:
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HM-50 was packaged aseptically in cryovials. The product is provided frozen and should be stored at -60°C or colder immediately upon arrival. For long-term storage, the vapor phase of a liquid nitrogen freezer is recommended. Freeze-thaw cycles should be avoided.
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Growth Conditions:
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Media:
Nutrient broth or Tryptic Soy broth or equivalent
Nutrient agar or Tryptic Soy agar or Tryptic Soy agar with 5% defibrinated sheep blood or equivalent
Incubation:
Temperature: 37°C
Atmosphere: Aerobic
Propagation:
1. Keep vial frozen until ready for use, then thaw.
2. Transfer the entire thawed aliquot into a single tube of broth.
3. Use several drops of the suspension to inoculate an agar slant and/or plate.
4. Incubate the tube, slant and/or plate at 37°C for 1 day.
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Disclaimers:
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You are authorized to use this product for research use only. It is not intended for human use. Use of this product is subject to the terms and conditions of the BEI Resources Material Transfer Agreement (MTA). The MTA is available on our Web site at www.beiresources.org. While BEI Resources uses reasonable efforts to include accurate and up-to-date information on this product sheet, neither ATCC® nor the U.S. Government makes any warranties or representations as to its accuracy. Citations from scientific literature and patents are provided for informational purposes only. Neither ATCC® nor the U.S. Government warrants that such information has been confirmed to be accurate. This product is sent with the condition that you are responsible for its safe storage, handling, use and disposal. ATCC® and the U.S. Government are not liable for any damages or injuries arising from receipt and/ or use of this product. While reasonable effort is made to ensure authenticity and reliability of materials on deposit, the U.S. Government, ATCC®, their suppliers and contributors to BEI Resources are not liable for damages arising from the misidentification or misrepresentation of products. |
References:
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1. Andersson, P., et al. “Persistence of Escherichia coli Bacteriuria Is Not Determined by Bacterial Adherence.” Infect. Immun. 59 (1991): 2915-2921. PubMed: 1879917.
2. Zdziarski, J., et al. “Host Imprints on Bacterial Genomes-Rapid, Divergent Evolution in Individual Patients.” PLoS Pathog. 6 (2010): e1001078. PubMed: 20865122.
3. Klemm, P., V. Hancock and M. A. Schembri. “Mellowing Out: Adaptation to Commensalism by Escherichia coli Asymptomatic Bacteriuria Strain 83972.” Infect. Immun. 75 (2007): 3688-3695. PubMed: 17502385.
4. Darouiche, R. O., et al. “Bacterial Interference for Prevention of Urinary Tract Infection: A Perspective, Randomized, Placebo-Controlled, Double-Blind Pilot Trial.” Clin. Infect. Dis. 41 (2005): 1531-1534. PubMed: 16231269.
5. Trautner, B. W., R. A. Hull and R. O. Darouiche. “Escherichia coli 83972 Inhibits Catheter Adherence by a Broad Spectrum of Uropathogens.” Urology 61 (2003): 1059-1062. PubMed: 12736047.
6. Hull, R. A., et al. “Virulence Properties of Escherichia coli 83972, a Prototype Strain Associated with Asymptomatic Bacteriuria.” Infect. Immun. 67 (1999): 429-432. PubMed: 9864249.
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Citation:
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Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH as part of the Human Microbiome Project: Escherichia coli, Strain 83972, HM-50."
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Biosafety Level:
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1
Appropriate safety procedures should always be used with this material. Laboratory safety is discussed in the following publication: U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control and Prevention, and National Institutes of Health. Biosafety in Microbiological and Biomedical Laboratories (BMBL). Current Edition. Washington, DC: U.S. Government Printing Office.
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