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NR-4670   Activated Epsilon Toxin, from Clostridium perfringens
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at no cost to registered researchers.
Description: Activated Epsilon Toxin, from Clostridium perfringens
Organism: Clostridium perfringens
Biosafety Level: 2
Availability Status: In Stock
Store at: -20°C or colder (Note: For long term storage, store at -80°C or colder.)
Contributor: AD O'Brien, JF Sinclair
Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Epsilon toxin is produced by strains of Clostridium perfringens (C. perfringens) that inhabit the intestinal tract of sheep and lambs. Intoxication results in enterotoxemia and neurological disorders and is usually fatal in certain livestock. The sequence of the gene for the epsilon toxin precursor protein has been reported (GenBank: M95206 and M80837). The structure of epsilon protoxin has been solved (PDB: 1UYJ).

Epsilon protoxin was purified from culture supernatants of C. perfringens ATCC ® 3626™ and activated by trypsin digestion. The protein is suitable for western blots and cytotoxicity assays.

Each vial of NR-4670 contains approximately 0.05 mg of epsilon toxin suspended in 0.1 M ammonium carbonate (pH 8.0).

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Activated Epsilon Toxin, from Clostridium perfringens, NR-4670."
Additional Information: NR-4670 Safety Data Sheet
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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