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NR-14823   Mycobacterium tuberculosis, Strain CDC1551, Whole Cell Lysate
(Antigen Preparations)
Price: All BEI Resources products are provided
at no cost to registered researchers.
Organism: Mycobacterium tuberculosis
Description: Mycobacterium tuberculosis, Strain CDC1551, Whole Cell Lysate
Biosafety Level: 1
Availability Status: In Stock
Store at: -80°C or colder
Contributor: K Dobos, NIH - TB Vaccine Testing and Research Materials Contract
Comments:

Quantity limit per order for this item is 1. This item can be ordered twice a year. Orders over this limit will be sent to NIAID for approval before shipment.

Mycobacterium tuberculosis, strain CDC1551 whole cell lysate contains proteins, lipids and carbohydrates present within the bacterial cell.

A culture was grown to late log phase in glycerol-alanine-salts medium and inactivated by gamma irradiation. Cells were suspended in PBS buffer containing 8 mM EDTA, proteinase inhibitors, DNase, and RNase, and disrupted by French Press until approximately 90% breakage was obtained. The lysate was centrifuged to pellet the unbroken cells and the cleared supernatant was removed. The protein content of the whole cell lysate was quantified using the BCA protein assay.

Each vial of whole cell lysate contains approximately 10 mg of protein provided in 10 mM ammonium bicarbonate.

Citations: Acknowledgment for publications should read "The following reagent was obtained through BEI Resources, NIAID, NIH: Mycobacterium tuberculosis, Strain CDC1551, Whole Cell Lysate, NR-14823."
Publications Citing
this Reagent:
Arlehamn, C. S. L., et al. "Memory T Cells in Latent Mycobacterium tuberculosis Infection are Directed against Three Antigenic Islands and Largely Contained in a CXCR3+ CCR6+ Th1 Subset." PLoS Pathog. 9 (2013): e1003130. PubMed: 23358848.
PERMITS
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  • Customers located in the state of Hawaii will need to contact the Hawaii Department of Agriculture to determine if an Import Permit is required. A copy of the permit or documentation that a permit is not required must be sent to ATCC in advance of shipment.

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