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The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the BEI Resources program and reagents through questions and answers. In order to use the Knowledge Base, enter a keyword or phrase that best describes the information of interest. More >>
   

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What is the importance of testing TPCK-treated trypsin lots from the same vendor when using it as a supplement in influenza virus growth in MDCK cells? How should it be tested?
TPCK-treated trypsin is available from several manufacturers. It is available in varying concentrations from different manufacturers, and some manufacturers offer the product based on specific activity, not concentration. Additionally, concentration will depend on purity. Therefore, it is important to determine the appropriate concentration of each lot of TPCK-trypsin used in the lab. To determine the appropriate concentration, the host cells should first be at 100% confluency before TPCK-treated trypsin is added. Then various concentrations should be tested on the 100% confluent host cell monolayer to determine the highest concentration that can be added to the cells without causing cytotoxicity to the host cell monolayer for the duration of the incubation period needed for growth of the virus. This testing should be conducted for each cell line that may be used with TPCK-treated trypsin to grow influenza virus, and must be repeated with each new lot of TPCK-treated trypsin.
Author: BEI Resources
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Updated on: 10/29/2014 2:53:55 PM
How do you adapt an egg grown Influenza virus to cell culture?
If adapting influenza virus from one propagation host to another propagation host, it is important to passage the virus at least three times in the new host to allow for adaptation. Each passage should be tested for virus growth, and it may also be important to check the titer of each passage to ensure that the virus is not being lost during adaptation to the new host. When changing the propagation host, it is also important to consider changes that may need to be made to the viral growth medium, temperature and cell confluency (if appropriate) based on the properties of the new host. For propagating influenza virus in tissue culture, the viral growth medium should not contain Fetal Bovine Serum (FBS), as serum will neutralize the activity of TPCK-treated trypsin.
Author: BEI Resources
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Updated on: 10/29/2014 2:56:37 PM
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Showing 1 to 5 of 9 Results12
BEI Resources Fungi Catalog is Growing!
  BEI Resources is working to expand the fungal catalog so that researchers have a broader range of organisms and reagents for their work.  BEI Resources currently has eight genus and species represented with several more currently going through the production pipeline.  BEI Resources i ...
Author: BEI Resources
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Updated on: 8/29/2014 9:49:43 AM
Housing and Maintenance of Larval Sand Fly Pots
Housing and Maintenance of Larval Pots SOP.docx
Author: BEI Resources
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Updated on: 2/20/2014 3:23:36 PM
MERS-CoV Now Available to Qualifed Labs
BEI Resources has the MERS-CoV virus (strain EMC/2012) available for distribution to qualified laboratories. This virus will require a BEI Level 3 registration. Middle East Respiratory Syndrome (MERS) is viral respiratory illness first reported in Saudi Arabia in 2012. It is caused by a coronavirus ...
Author: BEI Resources
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Updated on: 12/5/2013 10:58:28 AM
Sand Flies, Reduviids and Ticks are Here!
BEI Resources announces the addition of Sand Flies, Reduviids and Ticks for the research community. Catalog Number Vector Pathogen Competence   NR-43999 Phlebotomus papatasi, PPNS, Larval Leishmania major and Sand Fly Fever phleboviruses.   NR-44000 Phlebotomus papata ...
Author: BEI Resources
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Updated on: 12/4/2018 2:32:27 PM
SARS Virus Now Available for Researchers
We have available for the first time in the BEI Resources catalog, two SARS Coronaviruses (SARS-CoV) derived from the Urbani strain. NR-18925 is produced from a recombinant infectious clone, icSARS-CoV. NR-15418 is the mouse-adapted strain, Urbani v2163, developed by Dr. Barnard as a lethal model fo ...
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Updated on: 12/4/2018 2:36:38 PM
Showing 1 to 5 of 9 Results12
The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the BEI Resources Program.


Showing 1 to 5 of 3987 Results12345678910...>>
Kehn-Hall, Kylene / Narayanan, Aarthi / Lundberg, Lindsay / Sampey, Gavin / Pinkham, Chelsea / Guendel, Irene / Van Duyne, Rachel / (...) / Kashanchi, Fatah, Modulation of GSK-3ß Activity in Venezuelan Equine Encephalitis Virus Infection PLoS ONE, 7 (4), Apr 2012 doi:10.1371/journal.pone.0034761
Author: BEI Resources
Updated on: 8/20/2013 2:50:00 PM
Carpenter, Timothy S / Branda, Catherine S / Negrete, Oscar A / Carson, Bryan D, Identification of Critical Amino Acids within the Nucleoprotein of Tacaribe Virus Important for Anti-interferon Activity. Harmon, Brooke / Kozina, Carol / Maar, Dianna, The Journal of biological chemistry, 288 (12), p ...
Author: BEI Resources
Updated on: 8/16/2013 10:21:00 AM
Leppla, Stephen H., Chapter 281 - Anthrax Lethal Factor Handbook of Proteolytic Enzymes, Jan 2013 ISBN:9780123822192
Author: BEI Resources
Updated on: 8/16/2013 9:42:00 AM
Kingstad-Bakke, Brock / Brewoo, Joseph N. / Mai, Le Quynh / Kawaoka, Yoshihiro / Osorio, Jorge E., Effects of route and coadministration of recombinant raccoon poxviruses on immune responses and protection against highly pathogenic avian influenza in mice, Vaccine, 30 (45), p.6402-6408, Oct 2012 d ...
Author: BEI Resources
Updated on: 8/16/2013 9:47:00 AM
Turingan, Rosemary S. / Thomann, Hans-Ulrich / Zolotova, Anna / Tan, Eugene / Selden, Richard F., Rapid Focused Sequencing: A Multiplexed Assay for Simultaneous Detection and Strain Typing of Bacillus anthracis, Francisella tularensis, and Yersinia pestis, PLoS ONE, 8 (2), Feb 2013 doi:10.1371/jou ...
Author: BEI Resources
Updated on: 8/16/2013 9:49:00 AM
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