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The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the BEI Resources program and reagents through questions and answers. In order to use the Knowledge Base, enter a keyword or phrase that best describes the information of interest. More >>
   

Showing 1 to 5 of 5 Results
How are viruses propagated at BEI Resources?

 

Author: BEI Resources
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Updated on: 12/2/2013 3:34:51 PM
Will an egg grown virus grow in tissue culture?

Growing in tissue culture requires several passages to adapt the virus to tissue culture and it can alter the virus genetically. Some influenza viruses will not adapt to tissue culture growth.
Author: BEI Resources
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Updated on: 12/9/2013 11:48:30 AM
How should host cells be prepared for human metapneumovirus (hMPV) inoculation?
The hMPV isolates in BEI Resources are grown in LLC-MK2 Derivative cells (ATCC® CCL-7.1™). The cells should be adapted to low serum conditions by growing them in Opti-MEM Minimal Essential Medium supplemented with 2% fetal bovine serum and 2mM L-glutamine. It is also important to use low-passage number cells as they will need to be adapted to this low serum condition prior to inoculation. When the cells are ready to be used for inoculation, the monolayer should be rinsed with serum free medium or Dulbecco’s phosphate buffered saline prior to inoculation to remove any residual fetal bovine serum.
Author: BEI Resources
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Updated on: 11/20/2014 11:26:25 AM
How do you adapt an egg grown Influenza virus to cell culture?
If adapting influenza virus from one propagation host to another propagation host, it is important to passage the virus at least three times in the new host to allow for adaptation. Each passage should be tested for virus growth, and it may also be important to check the titer of each passage to ensure that the virus is not being lost during adaptation to the new host. When changing the propagation host, it is also important to consider changes that may need to be made to the viral growth medium, temperature and cell confluency (if appropriate) based on the properties of the new host. For propagating influenza virus in tissue culture, the viral growth medium should not contain Fetal Bovine Serum (FBS), as serum will neutralize the activity of TPCK-treated trypsin.
Author: BEI Resources
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Updated on: 10/29/2014 2:56:37 PM
Can you provide guidance on serum free adaptation of cell lines and protocols for A549, MRC-5, HEK-293 and HEK-293 T/17?

For more detailed information on serum free adaption of each individual cell line, please see click here.
Author: BEI Resources
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Updated on: 3/27/2020 10:28:20 AM
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SARS Virus Now Available for Researchers
We have available for the first time in the BEI Resources catalog, two SARS Coronaviruses (SARS-CoV) derived from the Urbani strain. NR-18925 is produced from a recombinant infectious clone, icSARS-CoV. NR-15418 is the mouse-adapted strain, Urbani v2163, developed by Dr. Barnard as a lethal model fo ...
Author: BEI Resources
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Updated on: 12/4/2018 2:36:38 PM
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The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the BEI Resources Program.

Showing 1 to 5 of 35 Results1234567
Galloway, Summer E. / Reed, Mark L. / Russell, Charles J. / Steinhauer, David A., Influenza HA Subtypes Demonstrate Divergent Phenotypes for Cleavage Activation and pH of Fusion: Implications for Host Range and Adaptation PLoS Pathogens, 9 (2), Feb 2013
Author: BEI Resources
Updated on: 8/14/2013 4:16:00 PM
Heinberg A, Siu E, Stern C, Lawrence E, Ferdig M, Deitsch K, Kirkman L. (2013). Direct evidence for the adaptive role of copy number variation on antifolate susceptibility in Plasmodium falciparum. Mol Microbiol.
Author: BEI Resources
Updated on: 8/21/2013 11:26:00 AM
Borucki, Monica K. / Allen, Jonathan E. / Chen-Harris, Haiyin / Zemla, Adam / Vanier, Gilda / Mabery, Shalini / Torres, Clinton / (...) / Slezak, Tom, The Role of Viral Population Diversity in Adaptation of Bovine Coronavirus to New Host Environments, PLoS ONE, 8 (1), Jan 2013 doi:10.1371/journal. ...
Author: BEI Resources
Updated on: 8/16/2013 9:40:00 AM
Yao Y, Barghava N, Kim J, Niederweis M, Marassi FM. (2011) Peptidoglycan recognition by Mycobacterium tuberculosis ArfA (Rv0899) reveals a link between acid adaptation and cell wall structure. J. Mol. Biol., in press.
Author: BEI Resources
Updated on: 1/5/2012 3:33:00 PM
Barr, Kelli L., and Benjamin D. Anderson. "Dengue viruses exhibit strain-specific infectivity and entry requirements in vitro." Virus Adaptation and Treatment 5 (2013): 1-9.
Author: BEI Resources
Updated on: 6/18/2014 9:03:44 AM
Showing 1 to 5 of 35 Results1234567

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