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The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the BEI Resources program and reagents through questions and answers. In order to use the Knowledge Base, enter a keyword or phrase that best describes the information of interest. More >>
   

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How are viruses propagated at BEI Resources?

 

Author: BEI Resources
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Updated on: 12/2/2013 3:34:51 PM
Will an egg grown virus grow in tissue culture?

Growing in tissue culture requires several passages to adapt the virus to tissue culture and it can alter the virus genetically. Some influenza viruses will not adapt to tissue culture growth.
Author: BEI Resources
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Updated on: 12/9/2013 11:48:30 AM
How should host cells be prepared for human metapneumovirus (hMPV) inoculation?
The hMPV isolates in BEI Resources are grown in LLC-MK2 Derivative cells (ATCC® CCL-7.1™). The cells should be adapted to low serum conditions by growing them in Opti-MEM Minimal Essential Medium supplemented with 2% fetal bovine serum and 2mM L-glutamine. It is also important to use low-passage num ...
Author: BEI Resources
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Updated on: 11/20/2014 11:26:25 AM
How do you adapt an egg grown Influenza virus to cell culture?
If adapting influenza virus from one propagation host to another propagation host, it is important to passage the virus at least three times in the new host to allow for adaptation. Each passage should be tested for virus growth, and it may also be important to check the titer of each passage to ens ...
Author: BEI Resources
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Updated on: 10/29/2014 2:56:37 PM
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SARS Virus Now Available for Researchers
We have available for the first time in the BEI Resources catalog, two SARS Coronaviruses (SARS-CoV) derived from the Urbani strain. NR-18925 is produced from a recombinant infectious clone, icSARS-CoV. NR-15418 is the mouse-adapted strain, Urbani v2163, developed by Dr. Barnard as a lethal model fo ...
Author: BEI Resources
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Updated on: 12/4/2018 2:36:38 PM
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The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the BEI Resources Program.

Showing 1 to 5 of 7 Results12
Galloway, Summer E. / Reed, Mark L. / Russell, Charles J. / Steinhauer, David A., Influenza HA Subtypes Demonstrate Divergent Phenotypes for Cleavage Activation and pH of Fusion: Implications for Host Range and Adaptation PLoS Pathogens, 9 (2), Feb 2013
Author: BEI Resources
Updated on: 8/14/2013 4:16:00 PM
Heinberg A, Siu E, Stern C, Lawrence E, Ferdig M, Deitsch K, Kirkman L. (2013). Direct evidence for the adaptive role of copy number variation on antifolate susceptibility in Plasmodium falciparum. Mol Microbiol.
Author: BEI Resources
Updated on: 8/21/2013 11:26:00 AM
Borucki, Monica K. / Allen, Jonathan E. / Chen-Harris, Haiyin / Zemla, Adam / Vanier, Gilda / Mabery, Shalini / Torres, Clinton / (...) / Slezak, Tom, The Role of Viral Population Diversity in Adaptation of Bovine Coronavirus to New Host Environments, PLoS ONE, 8 (1), Jan 2013 doi:10.1371/journal. ...
Author: BEI Resources
Updated on: 8/16/2013 9:40:00 AM
Yao Y, Barghava N, Kim J, Niederweis M, Marassi FM. (2011) Peptidoglycan recognition by Mycobacterium tuberculosis ArfA (Rv0899) reveals a link between acid adaptation and cell wall structure. J. Mol. Biol., in press.
Author: BEI Resources
Updated on: 1/5/2012 3:33:00 PM
Barr, Kelli L., and Benjamin D. Anderson. "Dengue viruses exhibit strain-specific infectivity and entry requirements in vitro." Virus Adaptation and Treatment 5 (2013): 1-9.
Author: BEI Resources
Updated on: 6/18/2014 9:03:44 AM
Showing 1 to 5 of 7 Results12

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