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BEI Resources Knowledge Base


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The Knowledge Base is an informational database assembled by our Support Specialists and Scientific Staff which provides additional knowledge about the BEI Resources program and reagents through questions and answers. In order to use the Knowledge Base, enter a keyword or phrase that best describes the information of interest. More >>
   

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How are viruses propagated at BEI Resources?

Viruses are grown in the same host and growth conditions as the original ones deposited by scientists in order to maintain virus population characteristics. If adaptation to another host is required, this is accompanied by creation of a new catalog number for the resulting new product.

Author: BEI Resources
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Updated on: 7/12/2022 11:12:26 AM
How do you adapt an egg grown Influenza virus to cell culture?
If adapting influenza virus from one propagation host to another propagation host, it is important to passage the virus at least three times in the new host to allow for adaptation. Each passage should be tested for virus growth, and it may also be important to check the titer of each passage to ensure that the virus is not being lost during adaptation to the new host. When changing the propagation host, it is also important to consider changes that may need to be made to the viral growth medium, temperature and cell confluency (if appropriate) based on the properties of the new host. For propagating influenza virus in tissue culture, the viral growth medium should not contain Fetal Bovine Serum (FBS), as serum will neutralize the activity of TPCK-treated trypsin.
Author: BEI Resources
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Updated on: 10/29/2014 2:56:37 PM
How should host cells be prepared for human metapneumovirus (hMPV) inoculation?
The hMPV isolates in BEI Resources are grown in LLC-MK2 Derivative cells (ATCC® CCL-7.1™). The cells should be adapted to low serum conditions by growing them in Opti-MEM Minimal Essential Medium supplemented with 2% fetal bovine serum and 2mM L-glutamine. It is also important to use low-passage number cells as they will need to be adapted to this low serum condition prior to inoculation. When the cells are ready to be used for inoculation, the monolayer should be rinsed with serum free medium or Dulbecco’s phosphate buffered saline prior to inoculation to remove any residual fetal bovine serum.
Author: BEI Resources
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Updated on: 11/20/2014 11:26:25 AM
Will an egg grown virus grow in tissue culture?

Growing in tissue culture requires several passages to adapt the virus to tissue culture and it can alter the virus genetically. Some influenza viruses will not adapt to tissue culture growth.

Author: BEI Resources
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Updated on: 12/9/2013 11:48:30 AM
Can you provide guidance on serum free adaptation of cell lines and protocols for A549, MRC-5, HEK-293 and HEK-293 T/17?

For more detailed information on serum free adaption of each individual cell line, please see click here.

Author: BEI Resources
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Updated on: 3/27/2020 10:28:20 AM
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SARS Virus Now Available for Researchers
We have available for the first time in the BEI Resources catalog, two SARS Coronaviruses (SARS-CoV) derived from the Urbani strain. NR-18925 is produced from a recombinant infectious clone, icSARS-CoV. NR-15418 is the mouse-adapted strain, Urbani v2163, developed by Dr. Barnard as a lethal model fo ...
Author: BEI Resources
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Updated on: 12/4/2018 2:36:38 PM
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The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the BEI Resources Program.


Showing 1 to 5 of 67 Results12345678910...>>
Weger-Lucarelli, J., et al., Evolution At Spike Position 519 in SARS-CoV-2 Facilitated Adaptation to Humans. 2024.
Author: BEI Resources
Updated on: 2/7/2024 2:40:25 PM
Guan, M., et al., Neu5Gc binding loss of subtype H7 influenza A virus facilitates adaptation to gallinaceous poultry following transmission from waterbirds but restricts spillback. bioRxiv, 2024: p. 2024.01. 02.573990.
Author: BEI Resources
Updated on: 2/7/2024 2:16:14 PM
Nayman, E.I., et al., Microbiome depiction through user-adapted bioinformatic pipelines and parameters. Journal of Medical Microbiology, 2023. 72(10): p. 001756.
Author: BEI Resources
Updated on: 11/20/2023 6:16:28 PM
Richner, J., et al., SARS-CoV-2 Bottlenecks and Tissue-Specific Adaptation in the Central Nervous System. 2023.
Author: BEI Resources
Updated on: 10/23/2023 9:11:58 AM
Jan, S., Vaccine-induced adaptive T cell immunity enhances protective responses against Coxiella burnetii. 2023, University of California, Irvine.
Author: BEI Resources
Updated on: 9/1/2023 9:24:10 AM
Showing 1 to 5 of 67 Results12345678910...>>
The following is a list of published abstracts and papers where the work was either facilitated, or directly supported, by the NIH HIV Reagent Program.



Showing 1 to 5 of 5 Results

Rawson, J.M., et al., Adaptation of HIV-1/HIV-2 Chimeras with Defects in Genome Packaging and Viral Replication. mBio, 2022: p. e02220-22.

Author: HIV
Updated on: 11/11/2022 12:49:25 PM

Real, F., et al., S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo. Nature Communications, 2022. 13(1): p. 1-16.

Author: HIV
Updated on: 11/11/2022 12:49:45 PM

Gao, C., et al., Differential recognition of oligomannose isomers by glycan-binding proteins involved in innate and adaptive immunity. Science Advances, 2021. 7(24): p. eabf6834.

Author: HIV
Updated on: 1/27/2023 11:29:07 AM

Real, F., et al., S100A8-mediated metabolic adaptation controls HIV-1 persistence in macrophages in vivo. Nature Communications, 2022. 13(1): p. 5956.

Author: HIV
Updated on: 3/13/2023 2:31:30 PM

Tumpach, C., et al., Adaptation of the intact proviral DNA assay to a nanowell based digital PCR platform. Journal of Virus Eradication, 2023. 9(2): p. 100335.

Author: HIV
Updated on: 9/1/2023 12:43:41 PM
Showing 1 to 5 of 5 Results